• Parallel evaluation of the synthesis of your protein of choice in different cell-free systems (feasibility study, proof of concept)
• Design and generation of optimal templates for prokaryotic and eukaryotic cell-free systems
• Protein production in prokaryotic and eukaryotic cell-free systems (e.g. translationally active lysates derived from cultured E.coli, yeast, insect, CHO and human cell lines)
• Tailor-made cell-free systems for customer-specific applications
• Production of posttranslationally modified proteins
• Optimization of reaction conditions (e.g. balancing the redox conditions, solubility and stability screens, increasing functionality by adjustment of the reaction environment, supplementing the reaction mixture with co-factors, chaperones, detergents, rare tRNAs)
• Radiolabeling of the protein(s) of interest for detailed quantitative and qualitative analysis (yield, homogeneity of the full-length product, detection of posttranslational modifications, e.g. glycosylations)
• Determination of the overall rate of protein breakdown by radiolabeling (targeted protein degradation, controlled perturbation of protein activity)
• Labeling of subunits, protein fractions, or purified proteins
• Introduction of mutations for protein engineering
• Protein assembly by design
• Introduction of non-canonical amino acids for conjugation (biorthogonal chemistry and Linkerology®)
• SOPs and test result specifications will be provided to the customer

For Research Use Only! Not for drug, food or household use, not for use in diagnostic procedures!