Protein Production Service

  • Parallel evaluation of the synthesis of your protein of choice in different cell-free systems (feasibility study, proof of concept)
  • Design and generation of optimal templates for prokaryotic and eukaryotic cell-free systems
  • Protein production in prokaryotic and eukaryotic cell-free systems (e.g. translationally active lysates derived from cultured E.coli, yeast, insect, CHO and human cell lines)
  • Tailor-made cell-free systems for customer-specific applications
  • Production of posttranslationally modified proteins
  • Optimization of reaction conditions (e.g. balancing the redox conditions, solubility and stability screens, increasing functionality by adjustment of the reaction environment, supplementing the reaction mixture with co-factors, chaperones, detergents, rare tRNAs)
  • Radiolabeling of the protein(s) of interest for detailed quantitative and qualitative analysis (yield, homogeneity of the full-length product, detection of posttranslational modifications, e.g. glycosylations)
  • Determination of the overall rate of protein breakdown by radiolabeling (targeted protein degradation, controlled perturbation of protein activity)
  • Labeling of subunits, protein fractions, or purified proteins
  • Introduction of mutations for protein engineering
  • Protein assembly by design
  • Introduction of non-canonical amino acids for conjugation (biorthogonal chemistry and Linkerology®)
  • SOPs and test result specifications will be provided to the customer

 

For Research Use Only! Not for drug, food or household use, not for use in diagnostic procedures!

Applications


For detailed information, ask the expert:
info@b4pt.de

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